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aav  (Vector Biolabs)


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    Vector Biolabs aav
    Aav, supplied by Vector Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/aav/product/Vector Biolabs
    Average 93 stars, based on 4 article reviews
    aav - by Bioz Stars, 2026-05
    93/100 stars

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    Glycyrrhizic acid inhibits SIRT3 protein expression in colorectal cancer cells. (a,b) Western blot analysis of SIRT3 protein expression in SW620 (a) and HT29 (b) cells after stimulating with 0, 5, 10 or 20 μmol/L glycyrrhizic acid for 24 hours. (c,d) Immunofluorescence analysis shows that SIRT3 is mainly expressed in the cytoplasm of SW620 (c) and HT29 (d) cells, and decreased expression of SIRT3 protein after stimulating with 20 μmol/L glycyrrhizic acid for 24 hours. Each test is repeated at least 3 times independently. * P < 0.05, ** P < 0.01 and *** P < 0.001 vs 0 μmol/L glycyrrhizic acid group.

    Journal: Bioengineered

    Article Title: Glycyrrhizic acid exhibits strong anticancer activity in colorectal cancer cells via SIRT3 inhibition

    doi: 10.1080/21655979.2021.2001925

    Figure Lengend Snippet: Glycyrrhizic acid inhibits SIRT3 protein expression in colorectal cancer cells. (a,b) Western blot analysis of SIRT3 protein expression in SW620 (a) and HT29 (b) cells after stimulating with 0, 5, 10 or 20 μmol/L glycyrrhizic acid for 24 hours. (c,d) Immunofluorescence analysis shows that SIRT3 is mainly expressed in the cytoplasm of SW620 (c) and HT29 (d) cells, and decreased expression of SIRT3 protein after stimulating with 20 μmol/L glycyrrhizic acid for 24 hours. Each test is repeated at least 3 times independently. * P < 0.05, ** P < 0.01 and *** P < 0.001 vs 0 μmol/L glycyrrhizic acid group.

    Article Snippet: We overexpressed SIRT3 in colorectal cancer cells by AAV-h-SIRT3 (AAV-223017, vectorbiolabs) lentivirus infection.

    Techniques: Expressing, Western Blot, Immunofluorescence

    Glycyrrhizic acid inhibits the proliferation of colorectal cancer cells by inhibiting SIRT3 in vitro. (a) Lentiviral infection increases the expression of SIRT3 protein in colorectal cancer cells using Western blot analysis. (b) SIRT3 overexpression increases decreased the cell viability of colorectal cancer induced by 20 μmol/L glycyrrhizic acid for 24 hours. (c,d) Wild-type and SIRT3 overexpressing colorectal cancer cells were cultured with 0 or 20 μmol/L glycyrrhizic acid for 2 weeks, representative cell clone images (c), and statistical comparison of the number of cell clones (d). Each test is repeated at least 3 times independently. ns P > 0.05 and *** P < 0.001 vs WT group, and ### P < 0.001 vs 20 μM GA group.

    Journal: Bioengineered

    Article Title: Glycyrrhizic acid exhibits strong anticancer activity in colorectal cancer cells via SIRT3 inhibition

    doi: 10.1080/21655979.2021.2001925

    Figure Lengend Snippet: Glycyrrhizic acid inhibits the proliferation of colorectal cancer cells by inhibiting SIRT3 in vitro. (a) Lentiviral infection increases the expression of SIRT3 protein in colorectal cancer cells using Western blot analysis. (b) SIRT3 overexpression increases decreased the cell viability of colorectal cancer induced by 20 μmol/L glycyrrhizic acid for 24 hours. (c,d) Wild-type and SIRT3 overexpressing colorectal cancer cells were cultured with 0 or 20 μmol/L glycyrrhizic acid for 2 weeks, representative cell clone images (c), and statistical comparison of the number of cell clones (d). Each test is repeated at least 3 times independently. ns P > 0.05 and *** P < 0.001 vs WT group, and ### P < 0.001 vs 20 μM GA group.

    Article Snippet: We overexpressed SIRT3 in colorectal cancer cells by AAV-h-SIRT3 (AAV-223017, vectorbiolabs) lentivirus infection.

    Techniques: In Vitro, Infection, Expressing, Western Blot, Over Expression, Cell Culture, Comparison, Clone Assay

    Glycyrrhizic acid blocks colorectal cancer cells in G1/G0 stage by inhibiting SIRT3 in vitro. (a,b) Flow cytometry analysis of cell cycle in SW620 (a) and HT29 (b) cells, Representative cell cycle diagrams are shown on the left, and statistical comparison cubes are shown on the right. (c,d) Western blot analysis of Cyclin D1, CDK2 and CDK4 protein expression in SW620 (c) and HT29 (d) cells after stimulating with 0 or 20 μmol/L glycyrrhizic acid for 24 hours. Each test is repeated at least 3 times independently. ** P < 0.01 and *** P < 0.001 vs WT group. # P < 0.05, ## P < 0.01 and ### P < 0.001 vs 20 μM GA group.

    Journal: Bioengineered

    Article Title: Glycyrrhizic acid exhibits strong anticancer activity in colorectal cancer cells via SIRT3 inhibition

    doi: 10.1080/21655979.2021.2001925

    Figure Lengend Snippet: Glycyrrhizic acid blocks colorectal cancer cells in G1/G0 stage by inhibiting SIRT3 in vitro. (a,b) Flow cytometry analysis of cell cycle in SW620 (a) and HT29 (b) cells, Representative cell cycle diagrams are shown on the left, and statistical comparison cubes are shown on the right. (c,d) Western blot analysis of Cyclin D1, CDK2 and CDK4 protein expression in SW620 (c) and HT29 (d) cells after stimulating with 0 or 20 μmol/L glycyrrhizic acid for 24 hours. Each test is repeated at least 3 times independently. ** P < 0.01 and *** P < 0.001 vs WT group. # P < 0.05, ## P < 0.01 and ### P < 0.001 vs 20 μM GA group.

    Article Snippet: We overexpressed SIRT3 in colorectal cancer cells by AAV-h-SIRT3 (AAV-223017, vectorbiolabs) lentivirus infection.

    Techniques: In Vitro, Flow Cytometry, Comparison, Western Blot, Expressing

    Glycyrrhizic acid inhibits the metastasis of colorectal cancer cells by inhibiting SIRT3 in vitro. (a,b) Transwell chamber was used to assess the invasion and migration ability of SW620 (a) and HT29 (b) cells. (c,d) Western blot analysis of MMP-9, N-cadherin and Vimentin protein expression in SW620 (c) and HT29 (d) cells after stimulating with 0 or 20 μmol/L glycyrrhizic acid for 24 hours. Each test is repeated at least 3 times independently. *** P < 0.001 vs WT group. ## P < 0.01 and ### P < 0.001 vs 20 μM GA group.

    Journal: Bioengineered

    Article Title: Glycyrrhizic acid exhibits strong anticancer activity in colorectal cancer cells via SIRT3 inhibition

    doi: 10.1080/21655979.2021.2001925

    Figure Lengend Snippet: Glycyrrhizic acid inhibits the metastasis of colorectal cancer cells by inhibiting SIRT3 in vitro. (a,b) Transwell chamber was used to assess the invasion and migration ability of SW620 (a) and HT29 (b) cells. (c,d) Western blot analysis of MMP-9, N-cadherin and Vimentin protein expression in SW620 (c) and HT29 (d) cells after stimulating with 0 or 20 μmol/L glycyrrhizic acid for 24 hours. Each test is repeated at least 3 times independently. *** P < 0.001 vs WT group. ## P < 0.01 and ### P < 0.001 vs 20 μM GA group.

    Article Snippet: We overexpressed SIRT3 in colorectal cancer cells by AAV-h-SIRT3 (AAV-223017, vectorbiolabs) lentivirus infection.

    Techniques: In Vitro, Migration, Western Blot, Expressing

    Glycyrrhizic acid induces the apoptosis of colorectal cancer cells by inhibiting SIRT3 in vitro. (a,b) 0 or 20 μmol/L glycyrrhizin stimulated colorectal cancer cells for 24 hours, flow cytometry was used to detect the apoptosis of colorectal cancer cells (a) and statistical comparison (b). (c) Western blot analysis of Bax and Bcl2 protein expression in colorectal cancer cells after stimulating with 0 or 20 μmol/L glycyrrhizic acid for 24 hours. (d) Elisa kit was used to determine the activity of caspase 3 in colorectal cancer cells after stimulating with 0 or 20 μmol/L glycyrrhizic acid for 24 hours. Each test is repeated at least 3 times independently. *** P < 0.001 vs WT group, and ### P < 0.001 vs 20 μM GA group.

    Journal: Bioengineered

    Article Title: Glycyrrhizic acid exhibits strong anticancer activity in colorectal cancer cells via SIRT3 inhibition

    doi: 10.1080/21655979.2021.2001925

    Figure Lengend Snippet: Glycyrrhizic acid induces the apoptosis of colorectal cancer cells by inhibiting SIRT3 in vitro. (a,b) 0 or 20 μmol/L glycyrrhizin stimulated colorectal cancer cells for 24 hours, flow cytometry was used to detect the apoptosis of colorectal cancer cells (a) and statistical comparison (b). (c) Western blot analysis of Bax and Bcl2 protein expression in colorectal cancer cells after stimulating with 0 or 20 μmol/L glycyrrhizic acid for 24 hours. (d) Elisa kit was used to determine the activity of caspase 3 in colorectal cancer cells after stimulating with 0 or 20 μmol/L glycyrrhizic acid for 24 hours. Each test is repeated at least 3 times independently. *** P < 0.001 vs WT group, and ### P < 0.001 vs 20 μM GA group.

    Article Snippet: We overexpressed SIRT3 in colorectal cancer cells by AAV-h-SIRT3 (AAV-223017, vectorbiolabs) lentivirus infection.

    Techniques: In Vitro, Flow Cytometry, Comparison, Western Blot, Expressing, Enzyme-linked Immunosorbent Assay, Activity Assay

    Glycyrrhizic acid inhibits SITR3 expression and the proliferation of colorectal cancer cells in nude mouse. (a-b) Subcutaneous injection of colorectal cancer cells to establish xenograft tumor models in nude mice. 3 weeks later, nude mice were euthanized, xenograft tumors were isolated, SIRT3 protein expression was detected by immunohistochemistry (a), and xenograft tumors were weighed for comparison (b). 8 mice per group. *** P < 0.001 vs WT group, and ### P < 0.001 vs 20 μM GA group.

    Journal: Bioengineered

    Article Title: Glycyrrhizic acid exhibits strong anticancer activity in colorectal cancer cells via SIRT3 inhibition

    doi: 10.1080/21655979.2021.2001925

    Figure Lengend Snippet: Glycyrrhizic acid inhibits SITR3 expression and the proliferation of colorectal cancer cells in nude mouse. (a-b) Subcutaneous injection of colorectal cancer cells to establish xenograft tumor models in nude mice. 3 weeks later, nude mice were euthanized, xenograft tumors were isolated, SIRT3 protein expression was detected by immunohistochemistry (a), and xenograft tumors were weighed for comparison (b). 8 mice per group. *** P < 0.001 vs WT group, and ### P < 0.001 vs 20 μM GA group.

    Article Snippet: We overexpressed SIRT3 in colorectal cancer cells by AAV-h-SIRT3 (AAV-223017, vectorbiolabs) lentivirus infection.

    Techniques: Expressing, Injection, Isolation, Immunohistochemistry, Comparison